Description
Luke A. Clifton
ISIS Pulsed Neutron and Muon Source, Rutherford Appleton laboratory, Didcot, Oxford, OX11 0QX
Without the large difference in neutron scattering length between hydrogen and deuterium neutron scattering would have not crossed over from the physical to the chemical and biological sciences to the extent seen over the previous decades. Indeed, for structural biology applications deuterium labelling is often key to unravelling the structure of biological complexes.
In this presentation I will discuss recent examples of deuterium labelling of complex membrane samples from experiments conducted in collaboration with the ISIS biology team. Emphasis will be placed on when sample deuterium labelling is best applied and where carful use of isotopic solution contrast is the best approach. Some examples of the parallels between the labelling required for neutron scattering techniques and other techniques will be discussed.
Primary author
Dr
Luke Clifton
(ISIS Spallation Neutron and Muon Source)
