Speaker
Ms
Maria Valldeperas
(Lund University)
Description
Nonlamellar lipid liquid crystalline phases have many potential applications, such as for drug delivery, protein encapsulation or crystallization.1-4 Lipid liquid crystalline sponge phase (L3) has not been very much considered in these applications, but have advantages in terms of its capacity of forming large aqueous pores able to encapsulate large bioactive molecules. This is more challenging to obtain with other reverse mesophases, such as reverse cubic (Q2) and reverse hexagonal (H2) phases. Basically, all of these reversed mesophases in excess aqueous solutions can be dispersed into colloidal stable particles dispersions.1,5,6 Here, we report a novel lipid system able to form highly swollen L3 phases, both in bulk and as nanoparticle dispersion. This system has been characterized by Dynamic Light Scattering (DLS), Cryo-Transmission Electron Microscopy (Cryo-TEM) and Small Angle X-ray Scattering (SAXS) to determine the size and shape of disperse particles as well as the liquid crystalline structure as a function of lipid composition. Water pores up to 13 nm were achieved, making the system suitable for entrapment of bioactive macromolecules such as proteins. Preliminary data on protein inclusion will also be presented.
| Topic Area / Session | Lipids |
|---|
Primary author
Ms
Maria Valldeperas
(Lund University)
Co-authors
Prof.
Dganit Danino
(Technion — Israel Institute of Technology)
Dr
Ellina Kesselman
(Technion — Israel Institute of Technology)
Dr
Justas Barauskas
(Camurus AB and Malmö University)
Ms
Maor Ram-On
(Technion — Israel Institute of Technology)
Ms
Małgorzata Wiśniewska
(University of Bergen)
Prof.
Tommy Nylander
(Lund University)
