Description
Robin Delhom(1)*, Valérie Laux(1), Michael Haertlein(1), Juliette Jouhet(3),
Hanna Wacklin(4), Giovanna Fragneto(1)
(1) Institut Laue-Langevin, 71 avenue des Martyrs, CS 20156, 38042 Grenoble, France
(2) Université Grenoble Alpes, 621 avenue centrale, 38400 Saint Martin d’Hères, France
(3) CEA, 17 avenue des Martyrs, 38000 Grenoble, France
(4) European Spallation Source, Brunnshög, 225 92 Lund, Suède
* email: delhomr@ill.fr
The lipid fraction of the cell membranes, previously thought to be mainly an inert support medium for proteins, is nowadays an extensively studied system [1-2]. The different lipid properties and the self-assembly of different molecular species have been shown to have an impact on biological processes [3]. In the neutron scattering community, the use of commercially available deuterated phospholipids has led to numerous publications over the years and this has increased our knowledge of lipid bilayers composed of one molecular species or a mixture of two or three different ones. However, in order to understand biological mechanisms occurring at cellular membranes, it is necessary to use a deuterated, biologically relevant lipid system. In this talk, I will describe (i) our lipid production method from yeasts, (ii) our observations of the effects of biological deuteration on the composition of biomembranes (Fig 1) as well as (iii) the structural analysis of the related supported lipid bilayers using neutron reflectivity.
Figure 1. Phospholipid composition of P. pastoris cells grown with different carbon sources.
PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; CL, cardiolipin or diphosphatidylglycerol. Data represent mean values. s.d (n = 2).
References:
[1] N.R. Yepuri et al, The synthesis of perdeuterated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC-d82) and the characterization of its lipid bilayer membrane structure by neutron reflectometry, ChemPlusChem (2015).
[2] Foglia, F., Lawrence, M. J., Deme, B., Fragneto, G., & Barlow, D. (2012). Neutron diffraction studies of the interaction between amphotericin B and lipid-sterol model membranes. Scientific Reports, 2, (2012) 1–6.
[3] A. de Ghellinck et al, Lipid polyunsaturation determines the extent of membrane structural changes induced by Amphotericin B in Pichia pastoris yeast, BBA, 10, (2015) 2317-2325.
[1]: https://indico.esss.lu.se/event/756/picture/14.jpg
Primary author
Mr
Robin DELHOM
(Institut Laue-Langevin, European Spallation Source ERIC and Grenoble-Alpes University)
